The role of vitamin B12 in thymine biosynthesis by chick bone marrow.
نویسندگان
چکیده
Studies with Lactobadlus tichmannii have indicated that vitamin Blz is required for the reduction of formate to the methyl group of thymine (1). Such a function is in agreement with other data implicating the vitamin in methyl group neogenesis and in thymine synthesis (2, 3). The present experiments were designed to study the role of vitamin Bls in thymine biosynthesis by bone marrow. The results to be presented demonstrate the involvement of Blz in the reduction of formate to the methyl group of thymine by chick bone marrow cells.
منابع مشابه
The role of vitamin B12 in thymine biosynthesis by Lactobacillus leichmannii.
The biochemistry of vitamin Biz has been the subject of several recent reviews (1, 2, 3). Evidence currently available indicates that the vitamin is required for the reduction of formate to methionine and choline methyl. Indirect evidence implicates vitamin Biz in thymine biosynthesis. Thymine will replace the Blz requirements in certain microorganisms (3) and will induce a reticulocyte respons...
متن کاملBiosynthesis of thymine and formyltetrahydrofolate by vitamin B12-deficient cells of Lactobacillus leichmannii.
A relationship between the functions of vitamin Blz and folic acid (pteroylglutamic acid) is implied by the hematological response induced by either of these vitamins in patients with pernicious anemia (1,2). This interrelationship between the two vitamins may arise because of separate functions at different steps in the formation of common products, such as thymidine, or because vitamin Bit ha...
متن کاملThe Role of Vitamin B,, in Thymine Biosynthesis by Lactobacillus leichmannii*
The biochemistry of vitamin Biz has been the subject of several recent reviews (1, 2, 3). Evidence currently available indicates that the vitamin is required for the reduction of formate to methionine and choline methyl. Indirect evidence implicates vitamin Biz in thymine biosynthesis. Thymine will replace the Blz requirements in certain microorganisms (3) and will induce a reticulocyte respons...
متن کاملSome Effects of Folk Acid and Vitamin B 12 on Nucleic Acid Metabolism in LactobaciZZus Zeichmannii*
Several lines of evidence implicate folic acid and vitamin I& in nucleic acid metabolism in various microorganisms. In some cases the exact effect has been carefully delineated. It is now well established that folic acid derivatives function in the insertion of l-carbon units into purines and into the 5-methyl group of thymine (1). It was shown by Shive et al. (2) that thymine would replace fol...
متن کاملThe effect of folate analogues and vitamin B12 on provision of thymine nucleotides for DNA synthesis in megaloblastic anemia.
The role of vitamin B12 in the folate dependent biosynthesis of thymidine nucleotides is controversial. In an attempt to clarify this, three methods have been used to assess the relative efficacy of vitamin B12 (hydroxocobalamin) and various folate analogues in titrated concentrations at correcting 'de novo' thymidylate synthesis by megaloblastic human marrow cells: (1) The deoxyuridine (dU) su...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 234 5 شماره
صفحات -
تاریخ انتشار 1959